by NanJing Agricultural University CRISPR-based genome editing in probiotics. Credit: BioDesign Research Probiotics, defined as live microorganisms beneficial to health, have shown promise in areas like anti-inflammatory activity and brain function, etc. However, challenges remain due to variability in tolerance and colonization among species and strains. Recent advances in genome engineering, including CRISPR-Cas, have facilitated the...
Willow Shah NevilleBy Willow Shah-Neville Photo/ShutterstockHIV CRISPR HIV continues to represent a global health burden, with the World Health Organization (WHO) estimating that, in 2022, there were 39 million people living with the virus. But there is still no effective cure for it. The only option patients have is antiretroviral therapy, which, although it represents...
by Bar-Ilan University Credit: Pixabay/CC0 Public DomainSevere combined immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for SCID patients...
by New York University Credit: Unsplash/CC0 Public Domain Artificial intelligence can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA, according to new research published in Nature Biotechnology. The study by researchers at New York University, Columbia University, and the New York Genome Center, combines a deep learning model with CRISPR screens to control...
MARCH 23RD, 2023 Researchers at Eindhoven University of Technology in the Netherlands have developed a sensitive diagnostic test for viral pathogens that is suitable for use in low-resource regions. The test is based on CRISPR proteins that can detect viral genetic material but also incorporates luciferase proteins, which are bioluminescent proteins that are naturally found...
AMERICAN CHEMICAL SOCIETY Most cancer diagnostic techniques rely on uncomfortable and invasive procedures, such as biopsies, endoscopies or mammograms. Blood samples could be a less unpleasant option, though only a few forms of the disease can currently be diagnosed this way. But now, researchers reporting in ACS Sensors have developed an easy-to-use method that can detect small amounts of...
by University of California, Los Angeles CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) For the first time, scientists have used CRISPR technology to insert genes that allow immune cells to focus their attack on cancer cells, potentially leaving normal cells unharmed and increasing...
by Walter and Eliza Hall Institute of Medical Research Generation and validation of pdCas9a-SAM construct in Eµ-Myc lymphoma cell lines. a Schematic representation of the pdCas9a-SAM lentiviral construct. b Western blot analysis for BCL-2 in manipulated Eµ-Myc lymphoma cell lines. EMRK-1184 or MRE-721 Eµ-Myc lymphoma-derived cell lines were transduced with pdCas9a-SAM only or pdCas9a-SAM plus Bcl-2...
by Eva Frederick, Massachusetts Institute of Technology Data for a new gene-function map are available for other scientists to use. “It’s a big resource in the way the human genome is a big resource, in that you can go in and do discovery-based research,” says Professor Jonathan Weissman. Credit: Jen Cook-Chrysos/Whitehead Institute The Human Genome...
BY JOCELYN KAISE, 1 JUN 2022 4:30 PM A version of this story appeared in Science, Vol 376, Issue 6597. Tools such as CRISPR that snip DNA to alter its sequence are moving tantalizingly close to the clinic as treatment for some genetic diseases. But away from the limelight, researchers are increasingly excited about an...
