Highlights Downregulation or inhibition of MCL-1 causes loss of OCT4 and NANOG expression MCL-1 downregulation or inhibition causes elongation of the mitochondrial network MCL-1 binds and modulates DRP-1 and OPA1 expression and activity Interactions between MCL-1 and DRP-1/OPA1 are disrupted by MCL-1 inhibitor Summary Human pluripotent stem cells (hPSCs) maintain a highly fragmented mitochondrial network, but the...