Using a mouse model of painful osteoarthritis, they found that blocking this pathway eliminates pain associated with osteoarthritis (OA) and results in a return to normal limb use.
The work, the first to find an association between this pathway and osteoarthritis pain, could lead to the development of new, effective pain treatments for people with OA, researchers say.
Over 32.5 million US adults suffer from painful OA, making it the most common joint disorder in the country. The incidence of OA is increasing, and while it can range in severity, OA can be associated with pain which limits mobility and function.
“There are currently very few effective and safe long-term ways to manage OA pain, which is chronic and often very debilitating,” says Duncan Lascelles, professor of translational pain research and management at North Carolina State University and co-corresponding author of the study in Frontiers in Neuroscience.
Previously, Lascelles, an expert in companion animal pain management, and his colleague, neurobiologist Santosh Mishra, observed increased levels (or upregulation) of the components of this signaling pathway in the joint fluid, blood, and sensory nerves of dogs with naturally occurring OA.
The components in question—the ligand, or binding molecule artemin, and its receptor GFRα3—were known to pain researchers, but had not been associated with OA pain signaling.
“When you feel pain, that’s the result of a molecule at the painful site interacting with a receptor on a sensory nerve, setting off a cascade of events within the nerve that lead to a signal being produced,” Lascelles says. “This signal travels along the nerve, and is interpreted as painful by the brain.”
“For acute pain, the artemin/GFRα3 system has been known to play a role, particularly in situations like cold hypersensitivity,” says Mishra, assistant professor of neuroscience and co-corresponding author of the work.
“However it had not been associated with pain in a chronic condition like OA. Observing upregulation of a particular molecule doesn’t necessarily mean it’s relevant in a particular condition, so we decided to explore whether this pathway was functionally involved in pain signaling in OA—that is, explore whether this signaling pathway was actually contributing to OA pain.”
In a mouse model of chemically induced OA the researchers found that GFRα3 was upregulated in the sensory nerves—just as it was in dogs with naturally occurring OA—versus a control group of healthy mice. Researchers then treated a subset of the OA mice with monoclonal antibodies designed to bind to GFRα3, preventing artemin from binding to GFRα3 and effectively blocking the pain signaling pathway.
Within two hours post-treatment with the antibodies, limb function had returned to normal levels in the treated mice, indicating that the artemin/GFRα3 pathway most likely plays an important role in OA pain.
“While this is a proof-of-concept study, the findings are encouraging and we hope to continue working to understand this pathway and its involvement in OA pain,” Mishra says.
“Although the work here is in a mouse model, it was based on robust observations in dogs with naturally occurring OA pain,” Lascelles says. “Because OA in dogs and humans is so similar, we believe our findings are highly relevant to both.
“Hopefully this work can lead to targeted drug therapies to relieve pain in both canine and human OA patients. While we cannot reverse the joint damage, we can hopefully alleviate suffering caused by pain, decreased mobility, and decreased ability to function.”
NC State’s Translational Research in Pain Program funded the work.
Source: NC State
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