These molecular machines have also proven effective at killing bacteria through tests on real infections.
Rice University chemist James Tour and his team successfully tested six variants of molecular machines. All of them punched holes in the membranes of gram-negative and gram-positive bacteria in as little as two minutes. Resistance was futile for bacteria that have no natural defenses against mechanical invaders. That means they are unlikely to develop resistance, potentially offering a strategy to defeat bacteria that have become immune to standard antibacterial treatments over time.
“I tell students that when they are my age, antibiotic-resistant bacteria are going to make COVID look like a walk in the park,” says Tour, professor of chemistry and of materials science and nanoengineering.
“Antibiotics won’t be able to keep 10 million people a year from dying of bacterial infections. But this really stops them.”
Coleaders of the study are Tour and Rice alumni Ana Santos and Dongdong Liu. Their findings appear in the journal Science Advances.
REFINING THE NANOSCALE DRILLS
Because extended exposure to UV can be damaging to humans, Tour’s lab has been refining its molecules for years. The new version gets its energy from still-blueish light at 405 nanometers, spinning the molecules’ rotors at 2 to 3 million times per second.
Other researchers have suggested that light at that wavelength has mild antibacterial properties of its own, but the addition of molecular machines supercharges it, says Tour, who suggested bacterial infections like those suffered by burn victims and people with gangrene will be early targets.
The machines are based on Nobel Prize-winning work by Bernard Feringa, who developed the first molecule with a rotor in 1999 and got the rotor to spin reliably in one direction. Tour and his team introduced their advanced drills in a 2017 Nature paper.
The lab’s first tests of the new molecules on burn wound infection models confirmed their ability to quickly kill bacteria, including methicillin-resistant Staphylococcus aureus, a common cause of skin and soft tissue infections that was responsible for more than 100,000 deaths in 2019.
The team achieved visible light activation by adding a nitrogen group. “The molecules were further modified with different amines in either the stator (stationary) or the rotor portion of the molecule to promote the association between the protonated amines of the machines and the negatively charged bacterial membrane,” says Liu.
MOLECULAR MACHINES KILL BACTERIA
The researchers also found the machines effectively break up biofilms and persister cells, which become dormant to avoid antibacterial drugs.
“Even if an antibiotic kills most of a colony, there are often a few persister cells that for some reason don’t die,” Tour says. “But that doesn’t matter to the drills.”
As with earlier versions, the new machines also promise to revive antibacterial drugs considered ineffective. “Drilling through the microorganisms’ membranes allows otherwise ineffective drugs to enter cells and overcome the bug’s intrinsic or acquired resistance to antibiotics,” says Santos.
The lab is working toward better targeting of bacteria to minimize damage to mammalian cells by linking bacteria-specific peptide tags to the drills to direct them toward pathogens of interest. “But even without that, the peptide can be applied to a site of bacterial concentration, like in a burn wound area,” Santos says.
Coauthors of the study are from Rice; the Health Research Institute of the Balearic Islands and the Son Espases University Hospital, Palma, Spain; and Tower Health, Reading, Pennsylvania.
The European Union’s Horizon 2020 research and innovation program, the Discovery Institute, and the Robert A. Welch Foundation supported the research.
Source: Rice University
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