By Deborah Balthazar At the University of Pittsburgh, testing to pick the best phage to fight infection-causing bacteria. UNIVERSITY OF PITTSBURGH Lynn Cole was in a never-ending cycle of getting recurrent blood infections. And no antibiotic drugs managed to kill off her zombie-like bacteria.“It just got so frustrating over the years because we couldn’t find the...
by University of Veterinary Medicine—Vienna Genome-wide CRISPR/Cas9-based loss-of-function screen identifies genetic dependencies of STAT3Y640F-driven cells a) Schematic illustration of a competitive proliferation assay. b) Competitive proliferation assays of clonal Cas9-expressing STATY640F-driven HPC7 or c) clonal Cas9-HPC7 empty vector cells expressing IRF670 and sgRNAs targeting either Myb (positive control) or the Rosa26 locus (negative control). d)...
