by University of Queensland (A–E) Live imaging of non-stimulated and stimulated (30 min, 20 Hz) Drosophila larval NMJ boutons expressing Atg8mCherry at endogenous levels in the absence of Ca2+ (A and A′), presence of Ca2+ (B and B′), presence of EGTA-AM (no Ca2+ in the buffer) (C), and presence of DMSO plus Ca2+ (D). Fluorescence intensities shown using scale (0–1292 gray value)...
