by PNAS Nexus Representative, low-field immunofluorescence sagittal image following treatment, fixation in formalin, and immunolabeling using a specific antibody against green-fluorescence protein (GFP). GFP-positive neurons positively transfected with guide RNA were identified in most brain regions including the olfactory bulb (OB), cortex, cerebellum, and numerous sub-cortical areas including the interpeduncular nucleus (IPN), a major connectome...
