Duchenne muscular dystrophy affects as many as one in every 3,500 boys It kills patients by their mid-30s by weakening the heart and breathing muscles Scientists have managed to edit DNA to strengthen weakened muscles Levels of a vital muscle protein rose to up to 92 percent of normal amounts Scientists have made a breakthrough...
Tag: <span>Crispr</span>
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Goodbye viral vectors, hello CRISPR
New technique successfully reprograms T cells without using viruses. Researchers from the University of California, San Francisco (CA, USA) have genetically reprogrammed T cells using a CRISPR gene-editing technology. The method could accelerate both research and cell therapy development for a range of diseases. The technique sees quantities of T cells, DNA and the CRISPR...
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CRISPR Study Shows Cas12a May Select DNA Target Sequences More Precisely Than Cas9
NEW YORK (GenomeWeb) – The Cas9 enzyme is known to have certain problems with binding to off-target DNA sequences, but for the most part, it is still considered to be the gold standard CRISPR enzyme for genome editing. However, in a new study in Molecular Cell today, researchers from the University of Texas at Austin...
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Potential DNA damage from CRISPR has been ‘seriously underestimated,’ study finds
From the earliest days of the CRISPR-Cas9 era, scientists have known that the first step in how it edits genomes — snipping DNA — creates an unholy mess: Cellular repairmen frantically try to fix the cuts by throwing random chunks of DNA into the breach and deleting other random bits. Research published on Monday suggests that’s only the tip...
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Biochemists discover cause of genome editing failures with hyped CRISPR system
New study sheds light on biology of most-used Cas9 target UNIVERSITY OF ILLINOIS AT CHICAGO Researchers from the University of Illinois at Chicago are the first to describe why CRISPR gene editing sometimes fails to work, and how the process can be made to be much more efficient. IMAGE: UIC RESEARCHERS SHOW PERSISTENT CAS9 BINDING TO A DOUBLE STRAND...
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Chinese scientists test CRISPR on monkeys and find no off-target mutations
With the first UK and US human trials using the potentially revolutionary CRISPR gene editing technique about to commence, several questions still hover over the ultimate safety of this system. A team of Chinese scientists has now conducted the first full assessment of the effects of CRISPR-Cas9 on non-human primates to ascertain whether it results...
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UCLA Researchers Use CRISPR Approach to Profile Genomic Variants in High-Throughput Manner
NEW YORK (GenomeWeb) – Measuring the functional effects of genomic variants in a high-throughput manner has been a challenge for researchers. But in a new study published today in Nature Genetics, a team from the University of California, Los Angeles detailed its efforts to develop a CRISPR-library-based approach for highly efficient and precise genome-wide variant engineering that...
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SALK SCIENTISTS MODIFY CRISPR TO EPIGENETICALLY TREAT DIABETES, KIDNEY DISEASE, MUSCULAR DYSTROPHY
Salk scientists modify CRISPR to epigenetically treat diabetes, kidney disease, muscular dystrophy Approach could also be applied to reversing aging and age-related diseases such as hearing loss and macular degeneration LA JOLLA—Salk scientists have created a new version of the CRISPR/Cas9 genome editing technology that allows them to activate genes without creating breaks in the...
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CRISPR toolbox gets two new molecular gadgets, boosting gene-editing
The acronyms might not be quite as catchy as CRISPR — since, really, what is? — but what new genetic tools dubbed REPAIR and ABE lack in whimsy they promise to make up in utility. These advances announced Wednesday solve two of the problems hobbling CRISPR, the revolutionary genome-editing technique: that its idea of...
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Scientists Establish New Nonviral Method for CRISPR Using Gold Nanoparticles
IN BRIEF CRISPR’s method of delivery has been raised as one limitation of the otherwise revolutionary technology. Now, a team of scientists has successfully tested a nonviral approach. GOLDEN TICKET CRISPR has the potential to revolutionize genetic engineering, but up until now, delivery methods have been something of a limiting factor. However, a new study has...