by Christin Senior, University of Central Florida Pipeline for calculation of TH-IR axon density. Six frames within each region of interest (ROI) were selected, converted to grayscale, thresholded using Otsu algorithm, skeletonized and the signal pixels were measured in each ROI to represent total length of axons travelled in each ROI. Credit: Scientific Reports (2023). DOI: 10.1038/s41598-023-27727-9 A team...
