Nocodazole can enrich cells in the G2/M, G1, and S phases of the cell cycle Treatment with nocodazole does not affect pluripotency maintenance hPSCs can efficiently form functional cell types after nocodazole treatment Nocodazole treatment allows genome-wide analyses of synchronous populations Summary Cell cycle progression and cell fate decisions are closely linked in human pluripotent stem cells (hPSCs). However, the study of these...
Tag: <span>human pluripotent cells</span>
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Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells
Highlights hPSCs can be differentiated into endothelial cells in 3D thermoreversible hydrogels The differentiation efficiency is similar to this in 2D cultures The global gene expression and phenotypes are similar to ECs made in 2D cultures Summary Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and...