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Unexpected finding in mucus clearance system may inform future pulmonary disease treatment
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Unexpected finding in mucus clearance system may inform future pulmonary disease treatment

by Alyssa Tomlinson, University of Maryland Generation and validation of MUC5B/AC-KO cultures. (A) Overview of the MUC5B/MUC5AC gene-targeting approach via lentivirus-mediated delivery of sgRNA and CRISPR-Cas9. Transduced and sorted cells were differentiated at air-liquid interface (ALI). Once fully differentiated, the mucus gel was collected by apically washing cultures. HEK293T, human embryonic kidney–293T; FACS, fluorescence-activated cell...