Nocodazole can enrich cells in the G2/M, G1, and S phases of the cell cycle Treatment with nocodazole does not affect pluripotency maintenance hPSCs can efficiently form functional cell types after nocodazole treatment Nocodazole treatment allows genome-wide analyses of synchronous populations Summary Cell cycle progression and cell fate decisions are closely linked in human pluripotent stem cells (hPSCs). However, the study of these...
Tag: <span>Pluripotent Stem Cell</span>
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Expansion and Purification Are Critical for the Therapeutic Application of Pluripotent Stem Cell-Derived Myogenic Progenitors
Highlights Tested CDM muscle differentiation protocol generates a heterogeneous population The population does not contribute to myofiber formation in vivo Regulated PAX7 expression allows expansion of homogeneous myogenic progenitors CDM iPAX7 myogenic progenitors are endowed with in vivo regenerative potential Summary Recent reports have documented the differentiation of human pluripotent stem cells toward the skeletal myogenic lineage...