Tag: <span>Pluripotent Stem Cells</span>

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Directed Differentiation of Human Pluripotent Stem Cells to Microglia

Highlights Efficient protocol to generate human microglia from PSCs iPSC-derived microglia have ramified morphology and motile processes Expression and cytokine profiles of iPSC-derived microglia resemble primary microglia iPSC-derived microglia can phagocytose beads and respond to ADP Summary Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and...

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CtIP-Specific Roles during Cell Reprogramming Have Long-Term Consequences in the Survival and Fitness of Induced Pluripotent Stem Cells

Highlights DNA end resection mechanism is hyper-activated during reprogramming CtIP protein levels increase during mouse and human cell reprogramming Loss of CtIP impairs mouse and human reprogramming efficiency Reprogramming without CtIP causes defects in iPSC self-renewal and differentiation Summary Acquired genomic instability is one of the major concerns for the clinical use of induced pluripotent...

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