by Flinders University Optimization of the VAIA method. A) Nitrocellulose membranes dotted with target antigens are concentrically placed in a VFD reactor housing a quartz tube (20 mm OD, 17.5 mm ID, 18.5 cm in length). The VFD reactor tube is tilted at 45° and rotated at 1500 rpm throughout the assay steps. At this speed the shear stress is...