by Thamarasee Jeewandara, Medical Xpress
Conceptual scheme of the dynamically adaptive molten fibril state for collagen based on the electro-assembly pathway. (A) Illustration of hierarchical assembly of collagen over length scales via diverse interaction mechanisms, reversible interactions of hierarchical assemblies allow for structural reconfigurations and adaptive functions, and irreversible interactions allow yielding terminal structures that maintain homeostasis in nature. (B) Illustration of the electro-assembly of collagen to create a molten fibril state and the mechanisms. (C) Roadmap that recapitulate the biomimetic structures and functions for collagen, electric signals input induce the dynamically adaptive intermediate assembly state formation, and selective external cues induce further functionalized assembly. Credit: Science Advances, 10.1126/sciadv.abl7506
Collagen is a building block that can be hierarchically assembled into diverse morphological structures that are dynamically adaptive in response to external cues. Materials scientists have limited capabilities of guiding the emergence of collagen’s hierarchical organization to recapture the richness of its biological structure and function in the lab. In a new report now published in Science Advances, Miao Lei, and a research team in materials science, medicine, and science and technology, in China and the U.S., described an electro-assembly pathway to build an intermediate molten fibril state for collagen. The intermediate state was structurally made of partially aligned and reversibly associated fibrils with limited hierarchical structure. They reversibly reconfigured the molten fibrils to offer dynamic properties such as stimuli-based stiffening, contracting, self-healing and self-shaping character and guided the molten fibrils to further assemble and recapitulate structural features of native collagen. The outcomes provide hitherto unidentified methods to tailor collagen-based biomedical materials.
Collagen as a biological building material
Structural proteins form important building blocks in biology with a variety of molecular interactions to cue their hierarchical assembly into complex morphological structures to regulate functional properties. Collagen is a classic example where triple helix molecules can be used to organize across a hierarchy of lengths and scales. Such assemblies can be connected via reversible interactions for structural reconfigurations and adaptive functional properties. For example, interactions seen with sea cucumbers describe dynamic cross-linking of collagen fibrils to reversibly tune their biomechanics and avoid predation. Such dynamics can also facilitate wound healing and remodeling via cell migration and self-organization. Examples of these structures in physiological environments include cross-linked collagen microfibrils of the transparent cornea, collagen fibrils of bone and teeth, skin and tough tendons. In this work, Lei et al. described the electro-assembly of an intermediate molten fibril state for collagen with a partially aligned fibril structure, but with limited hierarchical organization. They expect the electro-assembly of intermediate molten fibril states of collagen to provide previously unidentified opportunities to form collagen-based biomedical materials that mimic and incorporate structural properties of native collagen and collagen materials.
Electro-assembly and solution assembly of collagen I. (A) Picture of collagen I solution and scheme illustrates (B) the electro-assembly of EA-Col film with superior transparency and (C) solution assembly of SA-Col film with opaque appearance. Photo credit: M.L., East China University of Science and Technology. Scanning electron microscopy/transmission electron microscopy (SEM/TEM) micrographs, synchrotron two-dimensional small-angle x-ray scattering (2D SAXS) scattering patterns, and 1D SAXS profiles of (D to F) EA-Col and (G to I) SA-Col. A shift toward higher q upon EA-Col in (F) indicates its tighter packing structure, and the EA-Col has no D-banding characteristics compared to SA-Col. The calculated D-banding space of 62.7 nm in (I) is close to the measured value of 64.5 nm in (G). a.u., arbitrary units. (J) EA-Col visualized by methylene blue rapidly dissolved in 0.1 M HAc or 0.1 M urea (a hydrogen bond interrupter), respectively, indicating the internal reversible interactions in EA-Col. (K) SA-Col is quite stable in above solutions and only swells a little bit when exposed in HAc solutions, indicating the existence of irreversible interactions in SA-Col. Credit: M.L., East China University of Science and Technology. Science Advances, 10.1126/sciadv.abl7506Electro-assembly of collagen with a molten fibril state
Lei et al. first obtained acid-solubilized collagen from porcine skin to form a transparent molecular solution in acetic acid. They then induced the collagen solutions to self-assemble onto a titanium foil by imposing a cathodic voltage, when the pH of the solution was elevated. For comparison, the team prepared collagen films of approximately the same thickness via a conventional method. Such collagen hydrogel films maintained an opaque milky white appearance, designated as SA-Col, and remained semi-transparent. The high transparency of the electro-assembled collagen (designated EA-Col) could be stabilized via chemical crosslinking with benefits for biomedical applications including corneal implants that require long-term transparency. Lei et al. used scanning electron microscopy and transmission electron microscopy to show the microstructure of EA-Col films with dense organization and an aligned fibrous filamentous surface. They noted the organization of fibrils with a diameter of 10 nm. Then using nanostructure analysis, they performed synchrotron small-angle X-ray scattering to highlight the distinct, partially aligned fibril structure of the EA-Col network, in response to the imposed electric field.
The dynamic adaptability of molten fibril network to mechanical forces. (A) Visual evidence indicates that the molten fibril network (i.e., EA-Col) undergoes a plastic deformation, while static fiber network (i.e., SA-Col) shows an elastic deformation. Photo credit: M.L., East China University of Science and Technology. (B) The representative stress-strain curves and (C and D) cyclic loading-unloading curves show that the static fiber network undergoes little hysteresis between loading and unloading, while considerable hysteresis is observed with molten fibril network, which indicates that the static fiber network has elastic and molten fibril network has viscoelastic properties. (E) The increased Young’s modulus and (F) the decreased shape deformation ratio of molten fibril network during the 10 cycles’ loading and unloading indicate the internal structural reorganization ability of molten fibril network to adapt mechanical force. (G) The greater stress relaxation of molten fibril network further indicates that the process of mechanical adapting is accompanied with the dissipation of external stress. In contrast, the majority of the initially applied stress is stored in the static fiber network. Credit: Science Advances, 10.1126/sciadv.abl7506
Dynamic adaptability of molten fibril to mechanical forces
The scientists next studied the dynamic stability of molten fibrils to mechanical forces. They accomplished this by obtaining representative stress-strain curves to show the weakness of the molten fibril network, which underwent large deformation and gradual fracture. In contrast, the static fiber network had higher modulus, less deformation and underwent brittle fracture during stretch-release experiments. To further investigate the mechanical characteristics of molten fibrils, Lei et al. performed multicycle dynamic tensile loading measurements to note an increase in Young’s modulus during each consecutive loading cycle. They then investigated the mechanical variability of the collagen tissue structure to realize key physiological functions. For example, in nature, sea cucumbers can rapidly stiffen their connective networks to avoid predation by relying on the reversible regulation of interactions among adjacent collagen fibrils. Bioinspired by such interactions, Lei et al. incorporated Hofmeister ions that can influence hydrophobic (water-repelling) interactions to strengthen internal connections of the collagen fibril network. The collagen’s adaptive molten fibril state was highly responsive to the ions to adjust physical crosslinking and facilitate mechanical properties of molten fibril networks.
The strengthened molten fibril network provides dynamic mechanical functions in Vivo. (A) The illustration of a strengthened molten fibril band providing an initial high strength with dynamic relaxation over time in vivo for a decompression pulmonary artery surgery. (B) Photograph of the surgery process using 2 M Na2CO3 strengthened molten fibril band. Credit: M.L., East China University of Science and Technology. (C) Color Doppler ultrasound observation of the diameter of pulmonary artery over time, indicating that strengthened molten fibril band can provide a short-period mechanical restraints and then soften after 3 days to recover the normal pulmonary artery diameter and blood supply. Credit: Science Advances, 10.1126/sciadv.abl7506
Dynamic mechanical functions of the fibril networks in vivo and further experiments
Since the strengthening process of molten fibril networks by the Hofmeister effect is reversible, the team sought to develop biomedical concepts to highlight the adjustable and emergent properties of the construct, to thereby meet design objectives that can mechanically change with time. For example, during some surgical interventions, surgeons implant a band around an artery to constrict blood flow and protect a vulnerable downstream site from hypertension. While immediately after surgery this band should be strong, with time it should relax to allow greater blood flow. The ideal material to meet this medical requirement would therefore have mechanical properties that dynamically relax under conditions in vivo. A molten fibril network strengthened by a Hoffmeister salt could provide adequate strength to relax as the salt leached from the network, and after a period of time, the construct will be resorbed via preliminary biodegradation. Thereafter, the team tested the possibility of reconfiguring the structure and properties of molten fibril networks by regulating electrostatic interactions. Followed by experiments to highlight stimuli-contracting and self-healing properties of the material bioinspired by Cephalopods to create 3D complex shapes and form promising candidates for applications as bio-actuators, soft robots, and other intelligent biomimetic devices.
Biomimetic preparation of a terminal tissue tendon with highly aligned hierarchical fiber structure. (A) Illustration of the process to fabricate biomimetic tendon film: First, the aligned molten fibril network with 200% strain is treated by 0.1 M PBS to allow a highly ordered assembly under a constant external traction, and then UV/riboflavin is used to covalently cross-link this structure to obtain a biomimetic tendon film (with fiber and D-banding characteristics). Microscopic, SEM views and 2D SAXS patterns of (B and C) the biomimetic tendon film and (D and E) natural rabbit tendon suggest a highly similar hierarchical structure. (F) 1D SAXS plots prove the aligned structure and D-banding presented in the biomimetic tendon film. (G) Stress-strain curves and (H) the quantitative ultimate stress and Young’s modulus of dried samples indicate that the biomimetic tendon film has similar mechanical properties to the natural tendon tissue (P < 0.001). Credit: Science Advances, 10.1126/sciadv.abl7506Outlook
The experimental outcomes highlighted the formation of an intermediate molten fibril state during collagen electro-assembly, connected through responsive physical interactions. This property allowed the integration of external cues to the molten fibril network to generate biomimetic dynamic multi-functions. The team conducted further experiments to generate higher-order structural features, including the biomimicry of a tendon tissue to recapitulate the native microarchitecture of tendon collagen. In this way, Miao Lei and colleagues described an electro-fabrication method to form an intermediate molten fibril state for collagen. This state was adaptive and can be guided to form a higher ordered structure that integrates structural and functional properties of native collagen-based tissues. The team envision future work across three broad areas based on these outcomes, which include additional fundamental studies, additive manufacturing methods and the integration of collagen-based materials for regenerative medicine, including bone repair through bone tissue engineering and corneal or tendon replacement.
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