September 3, 2024
by Impact Journals LLC
FLNB and ACTC1 analysis. (A) mRNA FLNB expression (RT-qPCR) of fibroblasts in quiescence or after 24h of TGF-β-1 stimulation (Mean±SEM, n= 6 for young and old cells); (B) mRNA expression quantified by RT-qPCR after transfection with specific siRNA for FLNB (siFLNB) or with Scramble siRNA (siScramble), in quiescence or after a 24h-TGF-β-1 stimulation (Mean±SD, n=6 per type); (C) Collagen gel contraction (lattice) of old fibroblasts either from control cultures or after transfection with specific siFLNB or with siScramble during 120h (Mean±SD, n=6); (D) Wound closure over 96h as measured by % of cell confluency for analysis of migration of old control fibroblasts (CT) or after transfection with FLNB or siScramble (Mean±SD, n=6); (E) mRNA ACTC1 expression (RT-qPCR) of fibroblasts in quiescence or after 24h of TGF-β-1 stimulation (Mean±SEM, n= 6 for young and old cells); (F) mRNA expression quantified by RT-qPCR after transfection with specific siRNA for ACTC1 (siACTC1) or with Scramble siRNA (siScramble), in quiescence or after a 24h-TGF-β-1 stimulation (Mean±SD, n=6 per type); (G) Wound closure over 96h as measured by % of cell confluency for analysis of migration of old control fibroblasts (CT) or after transfection with siACTC1 or siScramble (Mean±SD, n=6); (H) Collagen gel contraction (lattice) of young fibroblasts either from control cultures or after transfection with specific siACTC1 or with siScramble during 120h (Mean±SD, n=6); p-value *<0.05, **<0.01, ***<0.001. Credit: Aging (2024). DOI: 10.18632/aging.206055
A new research paper titled “Proteomic and secretomic comparison of young and aged dermal fibroblasts highlights cytoskeleton as a key component during aging” has been published in Aging.
As noted in the abstract of this paper, dermal fibroblasts orchestrate the synthesis and degradation of extracellular matrix components, which is crucial for skin homeostasis. Alterations in the expression of components such as collagens and enzymes can lead to reduced mechanical cutaneous tension and impaired skin wound healing during aging.
In the study, researchers from Saint-Louis Hospital, Paris; Paris University, Paris Cité; Jacques-Monod Institute, Paris; and Clarins Laboratories, Pontoise, aimed to better understand the molecular alterations in fibroblasts during aging. They compared secretomic and proteomic signatures of fibroblasts from young (<35 years) and aged (>55 years) skin donors, in quiescent and TGF-stimulated conditions, using HLPC/MS.
This study revealed a significant decrease in fibroblast protein secretion with age and an enhancement of more than 60% of cytoplasmic protein accumulation.
“Our present data reinforce knowledge about an age-related alteration in the synthesis of major proteins linked to the migratory and contractile functions of dermal human fibroblasts,” the researchers note.
More information: Françoise Boismal et al, Proteomic and secretomic comparison of young and aged dermal fibroblasts highlights cytoskeleton as a key component during aging, Aging (2024). DOI: 10.18632/aging.206055
Provided by Impact Journals LLC
Leave a Reply